Figure 8: Representative optical microscopic images of hABMSCs after Alizarin red staining treatment with static condition (a1, b1) or at 10 min/day (a2, b2), 30 min/day (a3, b3), 60 min/day (a4, b4), 120 min/day (a5, b5), and 180 min/day (a6, b6) by OSS treatment in the absence of OM on 5 days and 10 days, respectively. OSS induction groups at 10, 30, and 60 min/day were intense compared to those of control (white arrows: mineral nodules stained in red). Representative microscopic images of hABMSCs after Von-Kossa staining with static condition (c1, d1) or at 10 min/day (c2, d2), 30 min/day (c3, d3), 60 min/day (c4, d4), 120 min/day (c5, d5), and 180 min/day (c6, d6) by OSS treatment in the absence of OM. Mineralized nodule as optical density measured after destaining treatment (C). OSS induction exposed at all of the groups was significantly different ( and ) among groups (, bar = 1 mm).