Rapid and Accurate Detection of Bacteriophage Activity against Escherichia coli O157:H7 by Propidium Monoazide Real-Time PCR

Figure 4

Selective quantification of survival E. coli O157:H7 R508N in bacteriophage treated samples. E. coli O157:H7 numbers were estimated by plate count (culturable), real-time PCR non-PMA (intact + compromised/damaged), and PMA-qPCR (intact only) methods. Samples were incubated at 37°C for 4 h. The mean ± standard deviation after exposure to four phages at each sampling time was compiled from two independent replicates.