Effects of Canonical NF-κB Signaling Pathway on the Proliferation and Odonto/Osteogenic Differentiation of Human Stem Cells from Apical Papilla
Figure 3
Effects of canonical NF-κB signaling pathway on the proliferation of SCAPs. (a) Cell proliferation in NF-κB pathway-activated and control groups was detected by MTT assay. (b) Flow cytometry analysis for SCAPs in NF-κB pathway-activated and untreated groups. The proliferation index (PI = S% + G2M%) in NF-κB pathway-activated group (26.52%) was significantly higher than that in control group (14.94%). (c) Images of scratch wounds in NF-κB pathway-activated and untreated groups at indicated time points. (d) Growth curves of NF-κB pathway-inhibited and untreated SCAPs. (e) Representative cell cycle distributions of NF-κB pathway-inhibited and untreated SCAP. The proliferation index (PI = S% + G2M%) in NF-κB pathway-activated group (8.90%) was obviously lower than that in control group (17.83%). (f) Cell motility of SCAPs in NF-κB pathway-inhibited and untreated group assessed by a scratch assay. Values were the means ± SD; ; ; ; . Scale bars = 100 μm.
