Association Analysis between g.18873C>T and g.27522G>A Genetic Polymorphisms of OPG and Bone Mineral Density in Chinese Postmenopausal Women

Table 1

PCR, CRS-PCR, and PCR-RFLP analysis used for genotyping OPG SNPs.

SNPs	Primer sequences	Annealing temperature (°C)	PCR amplification fragment (bp)	Region	Restriction enzyme	Genotype method	Genotype (bp)
g.18873C>T	5′-GACATCTCCATTAAGTGGACCG-3′	58.7	216	Exon 2	AciI	CRS-PCR	CC: 196, 20
	5′-GCTGCAGTATAGACACTCGTCAC-3′						CT: 216, 196, 20; TT: 216
g.27522G>A	5′-GAGCAGCTTCGTAGCTTGATG-3′	58.0	253	Exon 5	SphI	PCR-RFLP	GG: 173, 80
	5′-TTGTGAAGCTGTGAAGGAACC-3′						GA: 253, 173, 80; AA: 253

Note: SNPs, single-nucleotide polymorphisms. PCR, polymerase chain reaction; PCR-RFLP, PCR-restriction fragment length polymorphism; CRS-PCR, created restriction site-PCR. Underlined nucleotides mark nucleotide mismatches enabling the use of the selected restriction enzymes for discriminating sequence variations.