A Novel Role for Raloxifene Nanomicelles in Management of Castrate Resistant Prostate Cancer
Figure 8
Effect of free raloxifene or SMA-Ral treatment on cell migration and invasion. PC3 monolayer of cells was scratched and treated with either free raloxifene or SMA-Ral at 5 or 10 μM or controls (DMSO or SMA) and incubated for 20 h. Representative pictures were taken at h and at 20 h (a). For cell invasion, PC3 cells were treated with either free raloxifene or SMA-Ral at indicated concentrations. After 20 h-the cells migrating to the lower surface were fixed and stained with Diff Quick. Bars represent the mean ± SEM of three independent experiments (b). Conditioned mediums were collected from cultures following 24 and 48 h and analyzed by gelatin zymography (c). Bars indicate the relative MMP-9 activity in the conditioned media and represent the mean ± SEM of three independent experiments (d). Effects of free raloxifene and SMA-Ral on the formation of capillary-like structures by HUVEC. Cells were treated with either free raloxifene or SMA-Ral at 5 or 10 μM or controls (DMSO or SMA) for 24 h. Representative pictures were taken (e). PC3 cells cocultured with endothelial cells were treated with either free raloxifene or SMA-Ral at 10 μM or controls (DMSO or SMA) for 24 h. Representative pictures were taken (f). .
