Highly Effective Renaturation of a Streptokinase from Streptococcus pyogenes DT7 as Inclusion Bodies Overexpressed in Escherichia coli
Figure 2
Monoisotopic mass of three neutral identified peptides. (a) VNVNYEVSFVSETGDLDFTPLLR position 158–180 (a); (b) NQYHLTTLAVGDSLSSQELAAIAQFILSK position 181–208; (c) TNNTDLISEKYYVLK position 263–279; (d) NLDFRDLYDPR position 320–330 found in gi: 153807, streptokinase from Streptococcus pyogenes (GenBank, AAA26973) corresponding to ion scores of 102, 111, 73, and 51 with , respectively. (e) Alignment of four neutral identified peptides (4 peptides) with streptokinase from Streptococcus pyogenes AAA26973 (gi153807) and rSK from S. pyogenes DT07 (ACG50170).
