Research Article

Highly Effective Renaturation of a Streptokinase from Streptococcus pyogenes DT7 as Inclusion Bodies Overexpressed in Escherichia coli

Table 4

Effect of surfactants on streptokinase activity.

DetergentResidual activity (%) at the concentration (%)
0.51.01.52.0

Tween 20137.5 ± 2.6138.3 ± 1.8135.0 ± 2.3136.5 ± 2.6
Tween 80119.1 ± 2.8125.9 ± 2.6142.2 ± 1.5115.4 ± 2.2
Triton X-100150.3 ± 1.9140.6 ± 3.6143.6 ± 0.9126.6 ± 2.0

    Relative activity (%) after incubation for (h)
082448

Tween 20117.6 ± 1.5149.2 ± 1.3154.7 ± 1.221.9 ± 0.2
Tween 80121.9 ± 1.9151.9 ± 0.7154.4 ± 1.127.6 ± 0.2
Triton X-100119.3 ± 1.6162.6 ± 1.315.4 ± 0.118.1 ± 0.2