Research Article
Highly Effective Renaturation of a Streptokinase from Streptococcus pyogenes DT7 as Inclusion Bodies Overexpressed in Escherichia coli
Table 4
Effect of surfactants on streptokinase activity.
| Detergent | Residual activity (%) at the concentration (%) | 0.5 | 1.0 | 1.5 | 2.0 |
| Tween 20 | 137.5 ± 2.6 | 138.3 ± 1.8 | 135.0 ± 2.3 | 136.5 ± 2.6 | Tween 80 | 119.1 ± 2.8 | 125.9 ± 2.6 | 142.2 ± 1.5 | 115.4 ± 2.2 | Triton X-100 | 150.3 ± 1.9 | 140.6 ± 3.6 | 143.6 ± 0.9 | 126.6 ± 2.0 |
| | Relative activity (%) after incubation for (h) | 0 | 8 | 24 | 48 |
| Tween 20 | 117.6 ± 1.5 | 149.2 ± 1.3 | 154.7 ± 1.2 | 21.9 ± 0.2 | Tween 80 | 121.9 ± 1.9 | 151.9 ± 0.7 | 154.4 ± 1.1 | 27.6 ± 0.2 | Triton X-100 | 119.3 ± 1.6 | 162.6 ± 1.3 | 15.4 ± 0.1 | 18.1 ± 0.2 |
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