Comparative Metagenomic Analysis of Human Gut Microbiome Composition Using Two Different Bioinformatic Pipelines

Figure 3

Diversity analysis. Alpha diversity of the identified 16S rRNAs OTUs with MG-RAST and QIIME shows no significant variation between the two tools as measured using the observed species method (a) and Shannon index average scores (b). Also the single rarefaction curves obtained for each sample, as computed by the Chao1 estimator, show similar trends with the two tools (c). Beta diversity analysis among samples was carried out according to the Bray-Curtis metric; the same sample analyzed with the two tools appears to be distant, thus indicating individual differences [𝑃=0.028, 𝑅2=0.3; ADONIS] (d).

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