Comparative Metagenomic Analysis of Human Gut Microbiome Composition Using Two Different Bioinformatic Pipelines

Table 1

Summary of the taxonomic assignment data and of the algorithms available to obtain them in the MG-RAST and QIIME tools.

	Postfiltering reads*	Total distinct bacteria Families§	Bacteria families with >100 sequences^†	Available taxonomic assignment algorithms^‡	Default available 16S rRNA databases^±
MG-RAST	35,232	70	27	BLAT	Greengenes, LSU, SSU, M5RNA, RDP, no custom databases
QIIME	38,813	123	30	Rdp, Blast, Mothur, Rtax	Greengenes, custom databases

∗Number of reads obtained after the quality filtering step by the two bioinformatic pipelines; §number of distinct bacteria Families identified, as total number and ^†as the most represented Families with more than 100 sequences; ^‡taxonomy assignment algorithms available for both tools; ^±16S rRNA databases available for both tools.