Antioxidant and Anti-Inflammatory Effects of Selected Natural Compounds Contained in a Dietary Supplement on Two Human Immortalized Keratinocyte Lines
Figure 5
Effect of the combined treatment with all the seven supplement’s components on lipid peroxidation in keratinocytes exposed or not to prooxidant agents. Cells were treated simultaneously with all the components for 24 h and then exposed for 1 h to the prooxidant agents AAPH (25 mM) or t-BOOH (0.25 mM) or vehicle alone. Each component was used at the highest concentration reported in Section 2. Lipid peroxidation was evaluated spectrophotometrically as MDA production. Data are the means ± SE of a number of determinations ranging from 3 to 6. : significantly different from respective control (NCTC 2455: CT versus control, ; CT +AAPH versus AAPH, ; CT +t-BOOH versus t-BOOH, ; HaCaT: CT versus control, ; CT +AAPH versus AAPH, ; CT +t-BOOH versus t-BOOH, , two-tailed unpaired -test).
