Research Article

Antioxidant and Anti-Inflammatory Effects of Selected Natural Compounds Contained in a Dietary Supplement on Two Human Immortalized Keratinocyte Lines

Figure 6

Effect of α-T alone or combined with each of the other supplement’s components on lipid peroxidation in keratinocytes exposed or not to prooxidant agents. Cells were treated with α-T alone or combined with each of the other supplement’s components for 24 h and then exposed for 1 h to the prooxidant agents AAPH (25 mM) or t-BOOH (0.25 mM) or vehicle alone. Each component was used at the highest concentration reported in Section 2. Lipid peroxidation was evaluated spectrophotometrically as MDA production. Data are the means ± SE of a number of determinations ranging from 3 to 6. a: significantly different from respective control (); b: significantly different from respective control (), one-way ANOVA followed by Dunnett’s test.
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(a)
327452.fig.006b
(b)