Osteoponin Promoter Controlled by DNA Methylation: Aberrant Methylation in Cloned Porcine Genome

Figure 8

The CpG 13 and CpG 20 showed competition binding activity characteristics with c-Jun and c-Fos. The competition reduced the binding activity between transcription factors and labeled OPN EMSA probe. The amount of EMSA c-Jun and c-Fos antibody is 3μg. The EMSA probe is 30μg. Adding the Ab with a premix way can reduce the binding effects with the transcription factors. It is suggested that the premix with antibody blocks the access to its binding site (antibody-transcription factor-DNA).