Research Article

A Short-Term Incubation with High Glucose Impairs VASP Phosphorylation at Serine 239 in response to the Nitric Oxide/cGMP Pathway in Vascular Smooth Muscle Cells: Role of Oxidative Stress

Figure 1

Effects of a 120 min preincubation with 5 and 25 mmol/L glucose on total VASP expression and VASP phosphorylation at Ser 239 in the absence or in the presence of a 60 min incubation with the NO donor SNP (100 μmol/L). Blots are representative of six different experiments. The increase induced by SNP on VASP phosphorylation at both 5 and 25 mmol/L ( and , respectively) was lower at 25 than at 5 mmol/L ( ). SNP did not modify total VASP expression neither in the absence nor in the presence of high glucose.
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