A Short-Term Incubation with High Glucose Impairs VASP Phosphorylation at Serine 239 in response to the Nitric Oxide/cGMP Pathway in Vascular Smooth Muscle Cells: Role of Oxidative Stress
Figure 3
Effects of a 120 min preincubation with 5, 15, and 25 mmol/L glucose on VASP phosphorylation at Ser 239 in the absence or in the presence of a 60 min incubation with the cell-permeable cGMP analog 8-Br-cGMP (500 μmol/L). Blots are representative of six different experiments. 8-Br-cGMP induced a significant VASP phosphorylation at the three glucose concentrations ( for each of them). When values are expressed as percent of values at glucose 5 mmol/L, the extent of VASP phosphorylation induced by 8-Br-cGMP dose dependently decreased (ANOVA: ).
