A Short-Term Incubation with High Glucose Impairs VASP Phosphorylation at Serine 239 in response to the Nitric Oxide/cGMP Pathway in Vascular Smooth Muscle Cells: Role of Oxidative Stress
Figure 6
Effects of a 180 min incubation with 5 and 25 mmol/L glucose on ROS concentrations in the absence or in the presence of a 20 min preincubation with the PKC inhibitor chelerythrine (2.5 μmol/L), the NADPH-oxidase inhibitor apocynin (10 μmol/L), the mitochondrial electron transport chain complex inhibitor rotenone (10 µmol/L), the xanthine oxidase inhibitor allopurinol (50 µmol/L), and a mixture of SOD and catalase (300 U/mL/250 U/mL) (). ROS values in the presence of 25 mmol/L glucose were significantly higher than in the presence of 5 mmol/L glucose (, ). SOD + catalase, chelerythrine, and apocynin blunted the effects of glucose 25 mmol/L ( for each), whereas rotenone and allopurinol did not modify the high glucose effects ().
