Anti-Inflammatory Effects of Siegesbeckia orientalis Ethanol Extract in In Vitro and In Vivo Models
Figure 1
Effects of SOE on proinflammatory mediators production, iNOS expression, and cell viability in LPS-stimulated RAW264.7 macrophages. Cells were pretreated with 0 (control) or the indicated concentration of SOE for 1 h and then stimulated with 1 μg/mL LPS for 12 h (iNOS) or 24 h (proinflammatory mediators). The negative control (medium) is that cells were cultured with medium for the indicated time. (a) Production of IL-6 and TNF-α was measured by an ELISA kit. The nitrite concentration was analyzed using Griess reagent. (b) The expression of the iNOS protein was determined by Western blotting analysis. The iNOS level was quantified by densitometric analysis using the Quantity One software (Bio-Rad). (c) Cell viability was determined by MTT assay. Bar values are means ± SD of three independent experiments in these assays. A significant difference from the control (LPS alone) was indicated as , , or by Student’s -test.
