Gla-Rich Protein Is a Potential New Vitamin K Target in Cancer: Evidences for a Direct GRP-Mineral Interaction

Figure 1

Conformation-specific antibodies for γ-carboxylated (cGRP) and undercarboxylated (ucGRP) GRP forms. Amino acid sequence alignments of mature hGRP-F1 (hGRP) and synthetic peptides used as antigens for development of conformational c/ucGRP antibodies: cGRP29-42 and ucGRP31-54, respectively, and ucGRP29-42 and cGRP31-54 peptides used to test respective conformational specificity. GRP exons (Ex3, Ex4, and Ex5) are denoted in the corresponding amino acid sequence in gray scale; bold and underlined E in cGRP29-42 and cGRP31-54 indicate Gla residues.