Gla-Rich Protein Is a Potential New Vitamin K Target in Cancer: Evidences for a Direct GRP-Mineral Interaction
Figure 9
Both cGRP and ucGRP forms show in vitro mineral-binding affinity. (a) SDS-PAGE analysis of purified noncarboxylated recombinant human GRP-F1 (rhGRP) protein expressed in E. coli, detected with CBB. (b) SDS-PAGE analysis of non-mineral bound and mineral-bound proteins obtained from in vitro basic calcium-phosphate (BCP) protein mineral-binding assays, performed with purified rhGRP, sturgeon GRP (sGRP), bovine MGP (bMGP), and S6 ribosomal protein (S6). Relevant molecular weight markers (MW, kDa) are indicated on the left side of panels (a) and (b).
