Soybean Seeds: A Practical Host for the Production of Functional Subunit Vaccines
Figure 2
Molecular characterization of soy-mSEB events. (a) and (b) Duplex PCR of 8 T1 progeny from the indicated transformation events. WT: nontransgenic (negative control); +: plasmid DNA (positive control). Arrow shows position of amplified DNA fragments derived from mSEB and vegetative storage protein (VSP). Sizes of molecular weight markers are shown in base pairs. (c) and (d) Western blot of protein derived from the T1 progeny shown in (a) and (b). Arrow indicates soy-mSEB immunoreactive protein. Sizes of molecular weight standards are shown as kDa. (e) and (f) Western blots of T2 progeny from the indicated events. (g) and (h) Western blots of T3 progeny from the indicated events.
