A Modified Fluorimetric Method for Determination of Hydrogen Peroxide Using Homovanillic Acid Oxidation Principle

Figure 4

Stability of HVA dimer as reaction product in TCA treated (a) and TCA nontreated (b) H₂O₂ sample as a function of time. CAT (20 U) was incubated with the reaction mixture for different time periods (0–20 min) followed by TCA (5%, w/v) precipitation, neutralization with K₂HPO₄, and H₂O₂ quantification in HVA-HRP method.

(a)

(b)