Table 1: Differentially expressed proteins in CSM14.1 cells after 28 days of differentiation.

Acc. No.Entry nameProtein nameExpressionScoreMWpIQmScMixed

Structural proteins
Q6AYZ1TBA1C_RATTubulin alpha 1C-chainAbsent56505904.96411
P31000 VIME_RATVimentinUp205537575.062436
P70615LMNB1_RATLamin-B1Up188667945.162133
P60711 ACTB_RATActin, cytoplasmic 1Up110420525.29925
Q4V7C7ARP3_RATActin related protein 3Up168477835.611536
P85108TBB2A_RATTubulin beta 2A chainUp92502744.781440+
P48679 LMNA_RATLamin-ALandmark401745646.544851
Q63610TPM3_RATTropomyosin-alpha 3 chainLandmark202292174.751843
P09495TPM4_RATTropomyosin-alpha 4 chainLandmark253285494.661947

Regulating proteins
Q5BKC9NGEF_RATEphexin-1Absent55815275.8569
P62142PP1B_RATSerine/threonine-protein phosphatase PP1-beta catalytic subunitAbsent66379615.84519
O35763MOES_RATMoesinAbsent88678686.161016
P14668ANXA5_RATAnnexin A5Up288357794.932054
Q68FP1GELS_RATGelsolinUp227864135.762431
O35814STIP1_RATStress induced phosphoprotein 1Down160631586.41422
B3GNI6SEP11_RATSeptin-11Down102500056.24821
P38983RSSA_RAT40S ribosomal protein SA, laminin receptor 1, LRP/LR; laminin-binding protein precursor p40Down51329174.8417
P85515ACTZ_RATAlpha-centractinDown158427016.191140
Q99MZ8LASP1_RATLim and SH3 domain protein 1Landmark126303516.61928

Transport proteins
Q9Z2L0VDAC1_RATVoltage dependent anion selective channel protein 1Absent61308518.62419
P02793FRIL1_RATFerritin light chain 1Absent140207935.991158

Chaperones
Q5XHZ0TRAP1_RATHeat shock protein 75 kDa, mitochondrialAbsent118806396.561218
P28480TCPA_RATT-complex protein 1 subunit alphaAbsent115608355.86917
P52555ERP29_RATEndoplasmic reticulum protein ERp29Absent127286146.23831
Q66HD0ENPL_RATEndoplasmin, heat shock protein 90 kDa beta member 1Up190929984.722531
P06761GRP78_RAT78 kDa glucose-regulated protein, heat shock 70 kDa protein 5Up307724735.073856
P18418 CALR_RATCalreticulinLandmark192481374.331846
P63018HSP7C_RATHeat shock cognate 71 kDa proteinLandmark157710555.372239+
P63039CH60_RAT60 kDa heat shock protein, mitochondrial Landmark200610885.912042
P48721 GRP75_RATStress 70 proteinLandmark161740975.972541+
Q68FQ0TCPE_RATT-complex protein 1 subunit epsilonLandmark119599555.511828

Apoptosis
Q9QZA2PDC6I-RATProgrammed cell death 6-interacting proteinLandmark299971416.152832

Enzymes
Energy metabolism
P10719 ATPB_RATATP synthase subunit beta, mitochondrialAbsent146563185.192456+
P50516VATA_MOUSEVacuolar ATP synthase catalytic subunit AUp196686255.422336
P62815VATB2_RATVacuolar ATP synthase subunit B, brain isoformUp118568575.571328
P13803ETFA_RATElectron transfer flavoprotein subunit alpha, mitochondrialLandmark49352728.62413
Carbohydrate metabolism
P11980 KPYM_RATPyruvate kinase isoenzyme M1/M2 Absent175582946.631525
O88989 MDHC_RATMalate dehydrogenase, cytoplasmicDown69366316.16514
P07943ALDR_RATAldose reductaseDown103362306.261026
P04797G3P_RATGlyceraldehyde-3-phosphate dehydrogenaseLandmark111360908.14931
P04764ENOA_RATAlpha enolaseLandmark233474406.162458
Amino acid metabolism
P0C2X9AL4A1_RATDelta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrialAbsent166622867.141325
Q01205ODO2_RATDihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complexAbsent84492368.89921
Q9JLJ3AL9A1_RAT4-trimethylaminobutyraldehyde dehydrogenaseUp69545306.57921
P10860 DHE3_RATGlutamate dehydrogenase 1, mitochondrialLandmark192617198.052736
Fatty acid metabolism
P35571GPDM_RATGlycerol-3-phosphate dehydrogenase, mitochondrialAbsent123815496.181219
P15650ACADL_RATLong-chain specific acyl-CoA dehydrogenaseUp90482427.631018
Proteins against oxidative stress
P07632 SODC_RATSuperoxide dismutase [Cu-Zn]Absent134160735.88848
Q63081PDIA6_RATProtein disulfide isomerase A6Absent109485425823
Q8R4A1ERO1A_RATERO1-like protein alphaAbsent92548395.83719
P35704PRDX2_RATPeroxiredoxin 2Landmark91219415.34731
P11598PDIA3_RATProtein disulfide isomerase 3Landmark320570445.883356
P54001P4HA1_RATProlyl 4-hydroxylase subunit alpha-1Landmark237612025.632036
Proteasom, ubiquitin system
Q9JHW0PSB7_RATProteasome subunit beta type-7Absent61302508.13512
Q6AXR4HEXB_RATBeta-hexosaminidase subunit betaUp92618888.021123+

Signal transduction
P62260 1433E_RAT14-3-3 Protein epsilonLandmark127293264.631348+

Transcription
Q8VHV7HNRH1_RATHeterogeneous nuclear ribonucleoprotein HUp72494425.71128
P60123RUVB1_RATRuvB like 1, 49 kDa TATA box-binding protein-interacting proteinDown88505246.02619
Q9JMJ4PRP19_RATPre-mRNA-processing factor 19Down129556616.141328

Translation
Q4KM49SYYC_RATTyrosyl-tRNA synthetaseDown175594206.571629
P38983RSSA_RAT40S ribosomal protein SA, laminin receptor 1, LRP/LR; laminin-binding protein precursor p40Down51329174.8417
P05197EF2_RATElongation factor 2Landmark135961926.411822
Q6P9U8EIF3H_RATEukaryotic translation initiation factor 3 subunit HLandmark132400516.21036

Unknown function
Q4FZT0STML2_RATStomatin like protein 2UP85385048.74621

First column: Acc. No. (mascot identification). Second column: entry name (swiss-prot identification). Third column: protein title. Fourth column: expression (categories: absent, up, down, landmark). Fifth column: score (mascot-score). Sixth column: MW (theoretical protein mass [Da]). Seventh column: pI (theoretical isoelectric point). Eighth column: Qm (query matches). Ninth column: Sc (sequence covery [%]). Tenth column: mixed (mixed spot [+/−]).