The Proteome of the Differentiating Mesencephalic Progenitor Cell Line CSM14.1 In Vitro

Table 1

Differentially expressed proteins in CSM14.1 cells after 28 days of differentiation.

Acc. No.	Entry name	Protein name	Expression	Score	MW	pI	Qm	Sc	Mixed
Structural proteins
Q6AYZ1	TBA1C_RAT	Tubulin alpha 1C-chain	Absent	56	50590	4.96	4	11	−
P31000	VIME_RAT	Vimentin	Up	205	53757	5.06	24	36	−
P70615	LMNB1_RAT	Lamin-B1	Up	188	66794	5.16	21	33	−
P60711	ACTB_RAT	Actin, cytoplasmic 1	Up	110	42052	5.29	9	25	−
Q4V7C7	ARP3_RAT	Actin related protein 3	Up	168	47783	5.61	15	36	−
P85108	TBB2A_RAT	Tubulin beta 2A chain	Up	92	50274	4.78	14	40	+
P48679	LMNA_RAT	Lamin-A	Landmark	401	74564	6.54	48	51	−
Q63610	TPM3_RAT	Tropomyosin-alpha 3 chain	Landmark	202	29217	4.75	18	43	−
P09495	TPM4_RAT	Tropomyosin-alpha 4 chain	Landmark	253	28549	4.66	19	47	−
Regulating proteins
Q5BKC9	NGEF_RAT	Ephexin-1	Absent	55	81527	5.85	6	9	−
P62142	PP1B_RAT	Serine/threonine-protein phosphatase PP1-beta catalytic subunit	Absent	66	37961	5.84	5	19	−
O35763	MOES_RAT	Moesin	Absent	88	67868	6.16	10	16	−
P14668	ANXA5_RAT	Annexin A5	Up	288	35779	4.93	20	54	−
Q68FP1	GELS_RAT	Gelsolin	Up	227	86413	5.76	24	31	−
O35814	STIP1_RAT	Stress induced phosphoprotein 1	Down	160	63158	6.4	14	22	−
B3GNI6	SEP11_RAT	Septin-11	Down	102	50005	6.24	8	21	−
P38983	RSSA_RAT	40S ribosomal protein SA, laminin receptor 1, LRP/LR; laminin-binding protein precursor p40	Down	51	32917	4.8	4	17	−
P85515	ACTZ_RAT	Alpha-centractin	Down	158	42701	6.19	11	40	−
Q99MZ8	LASP1_RAT	Lim and SH3 domain protein 1	Landmark	126	30351	6.61	9	28	−
Transport proteins
Q9Z2L0	VDAC1_RAT	Voltage dependent anion selective channel protein 1	Absent	61	30851	8.62	4	19	−
P02793	FRIL1_RAT	Ferritin light chain 1	Absent	140	20793	5.99	11	58	−
Chaperones
Q5XHZ0	TRAP1_RAT	Heat shock protein 75 kDa, mitochondrial	Absent	118	80639	6.56	12	18	−
P28480	TCPA_RAT	T-complex protein 1 subunit alpha	Absent	115	60835	5.86	9	17	−
P52555	ERP29_RAT	Endoplasmic reticulum protein ERp29	Absent	127	28614	6.23	8	31	−
Q66HD0	ENPL_RAT	Endoplasmin, heat shock protein 90 kDa beta member 1	Up	190	92998	4.72	25	31	−
P06761	GRP78_RAT	78 kDa glucose-regulated protein, heat shock 70 kDa protein 5	Up	307	72473	5.07	38	56	−
P18418	CALR_RAT	Calreticulin	Landmark	192	48137	4.33	18	46	−
P63018	HSP7C_RAT	Heat shock cognate 71 kDa protein	Landmark	157	71055	5.37	22	39	+
P63039	CH60_RAT	60 kDa heat shock protein, mitochondrial	Landmark	200	61088	5.91	20	42	−
P48721	GRP75_RAT	Stress 70 protein	Landmark	161	74097	5.97	25	41	+
Q68FQ0	TCPE_RAT	T-complex protein 1 subunit epsilon	Landmark	119	59955	5.51	18	28	−
Apoptosis
Q9QZA2	PDC6I-RAT	Programmed cell death 6-interacting protein	Landmark	299	97141	6.15	28	32	−
Enzymes
Energy metabolism
P10719	ATPB_RAT	ATP synthase subunit beta, mitochondrial	Absent	146	56318	5.19	24	56	+
P50516	VATA_MOUSE	Vacuolar ATP synthase catalytic subunit A	Up	196	68625	5.42	23	36	−
P62815	VATB2_RAT	Vacuolar ATP synthase subunit B, brain isoform	Up	118	56857	5.57	13	28	–
P13803	ETFA_RAT	Electron transfer flavoprotein subunit alpha, mitochondrial	Landmark	49	35272	8.62	4	13	–
Carbohydrate metabolism
P11980	KPYM_RAT	Pyruvate kinase isoenzyme M1/M2	Absent	175	58294	6.63	15	25	–
O88989	MDHC_RAT	Malate dehydrogenase, cytoplasmic	Down	69	36631	6.16	5	14	–
P07943	ALDR_RAT	Aldose reductase	Down	103	36230	6.26	10	26	–
P04797	G3P_RAT	Glyceraldehyde-3-phosphate dehydrogenase	Landmark	111	36090	8.14	9	31	–
P04764	ENOA_RAT	Alpha enolase	Landmark	233	47440	6.16	24	58	–
Amino acid metabolism
P0C2X9	AL4A1_RAT	Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial	Absent	166	62286	7.14	13	25	–
Q01205	ODO2_RAT	Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex	Absent	84	49236	8.89	9	21	–
Q9JLJ3	AL9A1_RAT	4-trimethylaminobutyraldehyde dehydrogenase	Up	69	54530	6.57	9	21	–
P10860	DHE3_RAT	Glutamate dehydrogenase 1, mitochondrial	Landmark	192	61719	8.05	27	36	–
Fatty acid metabolism
P35571	GPDM_RAT	Glycerol-3-phosphate dehydrogenase, mitochondrial	Absent	123	81549	6.18	12	19	–
P15650	ACADL_RAT	Long-chain specific acyl-CoA dehydrogenase	Up	90	48242	7.63	10	18	–
Proteins against oxidative stress
P07632	SODC_RAT	Superoxide dismutase [Cu-Zn]	Absent	134	16073	5.88	8	48	–
Q63081	PDIA6_RAT	Protein disulfide isomerase A6	Absent	109	48542	5	8	23	–
Q8R4A1	ERO1A_RAT	ERO1-like protein alpha	Absent	92	54839	5.83	7	19	–
P35704	PRDX2_RAT	Peroxiredoxin 2	Landmark	91	21941	5.34	7	31	–
P11598	PDIA3_RAT	Protein disulfide isomerase 3	Landmark	320	57044	5.88	33	56	–
P54001	P4HA1_RAT	Prolyl 4-hydroxylase subunit alpha-1	Landmark	237	61202	5.63	20	36	–
Proteasom, ubiquitin system
Q9JHW0	PSB7_RAT	Proteasome subunit beta type-7	Absent	61	30250	8.13	5	12	–
Q6AXR4	HEXB_RAT	Beta-hexosaminidase subunit beta	Up	92	61888	8.02	11	23	+
Signal transduction
P62260	1433E_RAT	14-3-3 Protein epsilon	Landmark	127	29326	4.63	13	48	+
Transcription
Q8VHV7	HNRH1_RAT	Heterogeneous nuclear ribonucleoprotein H	Up	72	49442	5.7	11	28	–
P60123	RUVB1_RAT	RuvB like 1, 49 kDa TATA box-binding protein-interacting protein	Down	88	50524	6.02	6	19	–
Q9JMJ4	PRP19_RAT	Pre-mRNA-processing factor 19	Down	129	55661	6.14	13	28	–
Translation
Q4KM49	SYYC_RAT	Tyrosyl-tRNA synthetase	Down	175	59420	6.57	16	29	–
P38983	RSSA_RAT	40S ribosomal protein SA, laminin receptor 1, LRP/LR; laminin-binding protein precursor p40	Down	51	32917	4.8	4	17	–
P05197	EF2_RAT	Elongation factor 2	Landmark	135	96192	6.41	18	22	–
Q6P9U8	EIF3H_RAT	Eukaryotic translation initiation factor 3 subunit H	Landmark	132	40051	6.2	10	36	–
Unknown function
Q4FZT0	STML2_RAT	Stomatin like protein 2	UP	85	38504	8.74	6	21	–

First column: Acc. No. (mascot identification). Second column: entry name (swiss-prot identification). Third column: protein title. Fourth column: expression (categories: absent, up, down, landmark). Fifth column: score (mascot-score). Sixth column: MW (theoretical protein mass [Da]). Seventh column: pI (theoretical isoelectric point). Eighth column: Qm (query matches). Ninth column: Sc (sequence covery [%]). Tenth column: mixed (mixed spot [+/−]).