Biocatalytic Synthesis of Flavor Ester “Pentyl Valerate” Using Candida rugosa Lipase Immobilized in Microemulsion Based Organogels: Effect of Parameters and Reusability

Table 2

Full factorial Box-Behnken design for synthesis of pentyl valerate using MBGs in cyclohexane.

Block	Number	Enzyme concentration	Time	Substrate ratio	Wo	Solvent	Actual (%)	Predicted (%)
1	1	−1	−1	0	0	0	22.49	22.99
	2	1	−1	0	0	0	53.41	55.58
	3	−1	1	0	0	0	53.29	52.90
	4	1	1	0	0	0	88.96	86.25
	5	−1	0	−1	0	0	41.83	32.72
	6	1	0	−1	0	0	42.81	43.90
	7	−1	0	1	0	0	36.88	31.12
	8	1	0	1	0	0	88.39	87.78
	9	0	0	0	0	0	66.18	65.66
2	10	−1	0	0	−1	0	31.97	29.45
	11	1	0	0	−1	0	80.18	72.64
	12	−1	0	0	1	0	54.05	58.89
	13	1	0	0	1	0	78.15	82.89
	14	−1	0	0	0	−1	38.09	42.96
	15	1	0	0	0	−1	82.51	79.75
	16	−1	0	0	0	1	32.97	38.30
	17	1	0	0	0	1	71.86	72.25
	18	0	0	0	0	0	63.8	66.73
3	19	0	−1	−1	0	0	30.3	20.85
	20	0	1	−1	0	0	36.08	34.65
	21	0	−1	1	0	0	27.31	25.19
	22	0	1	1	0	0	70.85	71.96
	23	0	−1	0	−1	0	24.16	21.67
	24	0	1	0	−1	0	61.08	56.58
	25	0	−1	0	1	0	41.48	46.14
	26	0	1	0	1	0	71.83	71.79
	27	0	0	0	0	0	62.5	59.52
4	28	0	−1	0	0	−1	40.27	41.17
	29	0	1	0	0	−1	67.36	67.74
	30	0	−1	0	0	1	32.92	31.38
	31	0	1	0	0	1	68.21	64.01
	32	0	0	−1	−1	0	33.38	33.85
	33	0	0	1	−1	0	41.13	43.02
	34	0	0	−1	1	0	38.11	42.04
	35	0	0	1	1	0	73.67	74.53
	36	0	0	0	0	0	61.87	61.68
5	37	0	0	−1	0	−1	41.11	41.37
	38	0	0	1	0	1	59.57	56.45
	39	0	0	−1	0	1	37.76	42.85
	40	0	0	1	0	1	55.77	56.45
	41	0	0	0	−1	−1	57.6	60.57
	42	0	0	0	1	−1	84.34	75.99
	43	0	0	0	−1	1	41.33	49.72
	44	0	0	0	1	1	85.74	74.00
	45	0	0	0	0	0	64.48	69.86