Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells

Figure 1

Method of combined thermal and tensile stress conditioning for MC3T3-E1 monolayers. Cells were seeded on 6-well BioFlex plates with flexible culture substrate 16 hours before stress preconditioning. First, thermal stress was applied by heating in a water bath at 44°C for 4 or 8 minutes followed by 4-hour postheating incubation at 37°C and cyclic tensile stress conditioning protocols of equibiaxial 3% maximum elongation and 0.2 Hz (cycle of 10-second tension followed by a 10-second resting phase) using the Flexcell tension system. PT denotes the period of tensile stress treatment before cell analysis. The table summarizes the specific preconditioning protocols and associated assays performed.