Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells
Figure 2
Cell morphology as visualized by F-actin staining following stress conditioning. Cell morphology is shown in response to thermal stress ((b), (f), and (j)), tensile stress ((c), (g), and (k)), and combined thermal and tensile stress ((d, (h), and (l)). Static-cultured cells ((a), (e), and (i)) were used as a control. Varying positions within the well were imaged, including the center ((a)–(d)) and the edge under 100x ((e)–(h)) and 400x magnification ((i)–(l)). Yellow arrows denote the direction of tensile stress generated due to the loading post. Heating at 44°C for 8 minutes (postheating incubation = 4 hours); cyclic tension (equibiaxial 3%, 0.2 Hz, 10-second tension on/10-second rest, 24 hours).
