Influence of Heating and Cyclic Tension on the Induction of Heat Shock Proteins and Bone-Related Proteins by MC3T3-E1 Cells
Figure 6
MMP-9 gene expression and protein secretion by MC3T3-E1 cells following a single dose of thermal stress (44°C, 4 or 8 minutes) and cyclic tension (equibiaxial 3%, 0.2 Hz, 10-second tension on/10-second rest) alone or in combination. MMP-9 mRNA expression was measured with RT-PCR following heating for 4 minutes (a) or 8 minutes (b) alone or in combination with cyclic tension for 24 hours. MMP-9 protein secretion was measured with ELISA in response to heating for 4 minutes (c) or 8 minutes (d) alone or in combination with cyclic tension for 72 hours. denotes statistical significance between stress-treated and sham-treated control groups (). denotes statistical significance between individual thermal stress and combined thermal and tensile stress ( for (a); for (b)–(d)).
