BDNF Increases Survival and Neuronal Differentiation of Human Neural Precursor Cells Cotransplanted with a Nanofiber Gel to the Auditory Nerve in a Rat Model of Neuronal Damage
Figure 4
GFP+ HNPCs six weeks after transplantation with BDNF in PA gel (Group 3) (a). Cryostat section of dissected rat cochlea, auditory nerve, and brain stem following transplantation of HNPCs. Inset box in (a) shown at higher magnification in ((b)–(e)). Immunohistochemical staining was performed with DAPI (b) for cell nuclei, GFP (d) for transplanted cells, and TUJ1 (c) to verify differentiation. Double labeling (yellow) of GFP and TUJ1 indicates neuronal differentiation of the transplanted HNPCs. Double-labeled GFP, TUJ1 positive cells, and their arborisation of fibers can be identified in vicinity of the cochlear nucleus ((c)–(e)); arrowhead indicates GFP/TUJ1 double-labeled fiber. M: modiolus; AN: auditory nerve; BS: brain stem; CN: cochlear nucleus. Scale bar (a) 400 μm, ((b)–(e)) 50 μm.
