Review Article
The Design of a Quantitative Western Blot Experiment
Table 2
Computational analysis of densitometric data (*density data from a control sample loaded in the first lane of each gel/blot for a given study).
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Biological replicates | Sample | Density (volume) | Target protein (TP) | Loading control (LC) | Normalized density to LC (NDL) (TP* LC/LC*) | Fold difference (NDL/NDL*) |
| M1 | | 8 | 4* | | | M1 | | 45 | 5 | | | M1 | | 90 | 6 | | | M2 | | 6 | 5 | | | M2 | | 40 | 5 | | | M2 | | 88 | 7 | | | M3 | | 10 | 4 | | | M3 | | 48 | 6 | | | M3 | | 92 | 6 | | |
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