Research Article

Nodal Promotes the Self-Renewal of Human Colon Cancer Stem Cells via an Autocrine Manner through Smad2/3 Signaling Pathway

Figure 6

Protein expression of NODAL and its receptors in CD44- and CD24-negative colon cancer cells. (a) Immunocytochemistry showed NODAL translation in CD24-negative HCT116 cells. Scale bars = 20 μm. (b) Western blots displayed NODAL expression in HCT116 cells (lane 1) and CD24-negative HCT116 cells (lane 2). ACTB was used as a loading control of total proteins. (c-d) Immunocytochemistry showed the expression of ALK-4 (c) and ALK-7 (d) in CD24-negative HCT116 cells. Scale bars in (c)-(d) = 20 μm.
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