The Structural Modeling of the Interaction between Levofloxacin and the Mycobacterium tuberculosis Gyrase Catalytic Site Sheds Light on the Mechanisms of Fluoroquinolones Resistant Tuberculosis in Colombian Clinical Isolates

Table 3

OFL, LVX, and MFX MIC for ten isolates fluoroquinolones resistant X: last dilution with growth.

Isolate

Mutation in GyrA

Mutation in GyrB

OFL MIC mg/L

Cut-off MIC for resistance

LVX MIC mg/L

Cut-off MIC for resistance

MFX MIC mg/L

Cut-off MIC for resistance

0.25

0.5

1

2

4

8

16

0.25

0.5

1

2

4

8

16

0.25

0.5

1

2

4

8

16

TBR-49

Ala90Val

NM

X

>2.0

X

>2.0

X

>0.5

TBR-67

Asp94Gly

NM

X

X

X

TBR-73

NM

NM

X

X

X

TBR-31

Ala90Val

NM

X

X

X

TBR-103

NM

NM

X

X

X

TBR-18

NM

NM

X

X

X

TBR-107

NM

NM

X

X

<0.25

TBR-111

Asp94Gly

NM

X

X

X

TBR-102

Asp94Gly

NM

X

X

X

TBR-176

Asp94Gly

NM

X

X

X