Research Article
Preparation of North American Type II PRRSV Infectious Clone Expressing Green Fluorescent Protein
Table 1
Primers for amplifying gfp gene fused with PRRSV Nsp2 gene sequence.
| Primers | Sequence of primers () | Sizes/bp | Location in the plasmids |
| F1 | CTACTATCCT GCACAAGGTG |
355 | 2351~2370 (FL12) | R1 | CAGGCCGCTCTCGTCGCTCTCTGGCAAGCTCTTGACAGACTTTG | 9854~9875 (pCDNA-EF1-GFP) 2662~2684 (FL12) |
| F2 | CAAAGTCTGTCAAGAGCTTGCCAGAGAGCGACGAGAGCGGCCTG |
800 | 2662~2684 (FL12) 9854~9875 (pCDNA-EF1-GFP) | R2 | GCGGGGACAGGTTTGTTCCCGCGAGATCCGGTGGAGCC | 2684~2704 (FL12) 10589~10607 (pCDNA-EF1-GFP) |
| F3 | GGCTCCACCGGATCTCGCGGGAACAAACCTGTCCCCGC | 840 | 10589~10607 (pCDNA-EF1-GFP) 2684~2704 (FL12) | R3 | GGTGTCTCGAGTATCATCTTTG | 3481~3502 (FL12) |
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