Preparation of North American Type II PRRSV Infectious Clone Expressing Green Fluorescent Protein

Table 2

Tm and extension time for amplifying the corresponding segment.

PCR fragments	Annealing temperature (°C)	Extension time (S)
Frag-1	56	30
Frag-2	58	60
Frag-3	58	60
Frag-4	58	90
Nsp2-GFP frag	60	120