Functional Analyses of c.2268dup in Thyroid Peroxidase Gene Associated with Goitrous Congenital Hypothyroidism

Figure 5

Western blot and Image-J densitometric analyses. Microsomal protein samples were first tested with anti-TPO antibody followed by anti-beta actin antibody (loading control). Equal amount of microsomal protein (20 μg) for each sample (100 ng for the positive control) was used. Lane 1: PageRuler prestained protein ladder; lane 2: normal area of thyroid tissue of proband’s sister (III-1); lane 3: lesion area of thyroid tissue of proband’s sister (III-1); lane 4: normal area of thyroid tissue of proband; lane 5: lesion area of thyroid tissue of proband; lane 6: normal and lesion areas (10 μg each) of thyroid tissue of proband’s sister (III-1); lane 7: normal and lesion areas (10 μg each) of thyroid tissue of proband (III-2); lane 8: recombinant fragment of human thyroid peroxidase (positive control).