Alcohol Induced Mitochondrial Oxidative Stress and Alveolar Macrophage Dysfunction
Figure 2
Chronic EtOH ingestion induced ROS production in mAMs. mAMs were collected from control and EtOH-fed mice (). The collected mAMs were cultured and treated with mitoT for 24 hrs. Cells were stained with CM-H2DCFDA (a) for cellular ROS and mitoSOX (b) for mitochondrial superoxide. Computer analysis of confocal microscopic images was used to quantify ROS generation. All values are expressed as mean ± SE and normalized to control conditions. for EtOH versus control and for 5d EtOH versus EtOH + mitoT.
