Alcohol Induced Mitochondrial Oxidative Stress and Alveolar Macrophage Dysfunction
Figure 8
EtOH-induced apoptosis was blocked by mitoT. Cultured MH-S cells were either untreated (none) or EtOH treated (1, 3, or 5d, 0.2%, ). Cells were then incubated with the Dead Cell Apoptosis Kit with Annexin V Alexa Fluor 488 and Propidium Iodide (PI) (Invitrogen, Carlsbad, CA) before being analyzed by flow cytometry. A representative flow image for MH-S cells without EtOH treatment is shown in (a). A representative flow image for MH-S cells treated with EtOH for 5 days is shown in (b). The fluorescence mean intensity for live, early apoptotic (Annexin V positive), late apoptotic (positive for Annexin V and cytosolic shrinkage), and necrotic cells (positive for propidium iodide) is shown in (c). During the last 24 hr of EtOH treatment, mitoT was also added to some MH-S cells before assessment of Annexin V staining (d). All values are expressed as mean ± SD and normalized to the untreated condition (). For (c), * denotes for EtOH versus none and # denotes for EtOH versus EtOH + mitoT. For (d), * denotes compared to the no treatment group.
