Polymorphisms at Amino Acid Residues 141 and 154 Influence Conformational Variation in Ovine PrP

Figure 4

ELISA reactivity of metal ion-treated ovine recombinant PrP. Ovine recombinant PrP ALRQ (a, c, e, g) or ALHQ (b, d, f, h) was treated with water (black square) or either 0.2 mM (black circle) or 2 mM (black triangle) copper (a–d) or manganese (e–h) at 37°C as described in Section 2. Serial 2-fold dilutions of recombinant PrP were analysed by direct ELISA for reactivity with anti-PrP monoclonal antibody FH11 (a, b, e, f) or V47 (c, d, g, h). The data shown are the means of triplicate wells ± SD.

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