Methylation-Associated Gene Silencing of RARB in Areca Carcinogens Induced Mouse Oral Squamous Cell Carcinoma

Table 1

Primer sets used for RT-PCR, MS-PCR, and bisulfite sequencing.

Primer sets	Sense primer (5′→3′)	Antisense primer (5′→3′)	Tm (°C)	PCR size (bp)
RARB-Hu-RT	AGGAGACTTCGAAGCAAG	GTCAAGGGTTCATGTCCTTC	60	771
Dnmt1-Hu-RT	TACCTGGACGACCCTGACCTC	CGTTGGCATCAAAGATGGACA	60	102
Dnmt3a-Hu-RT	TATTGATGAGCGCACAAGAGAGC	GGGTGTTCCACCCTAACATTGAG	64	110
Dnmt3b-Hu-RT	GGCAAGTTCTCCGAGGTCTCTG	TGGTACATGGCTTTTCGATAGGA	62	112
RARB-Mo-M	GGATTAGAGTTTTCGTGCGTCG	TACCCCGCCGATACCCAAACG	65	90
RARB-Mo-U	GGATTAGAGTTTTTGTGTGTTG	TACCCCACCAATACCCAAACA	62	90
RARB-Mo-BS	CCACCCAACTCCATCAAACTC	CCATACAATCAAACATAATCTC	58	476
RARB-Hu-M	ATGTCGAGAACGCGAGCGATTC	CTCGACCAATCCAACCGAAACG	64	151
RARB-Hu-U	GGATGTTGAGAATGTGAGTGATTT	TACTCAACCAATCCAACCAAAACA	62	155
RARB-Hu-BS	GTGTGATAGAAGTAGTAGGAAG	GTGATAGAAGTGGTAGGAAG	55	401

*Hu: human, Mo: mouse, RT: real-time RT-PCR, M: methylated set, U: unmethylated set, BS: bisulfite sequencing.