Lentiviral Protein Transduction with Genome-Modifying HIV-1 Integrase-I-PpoI Fusion Proteins: Studies on Specificity and Cytotoxicity

Figure 1

Interaction of different IN proteins with the I-PpoI site on chromosome 1. The binding of IN-I-PpoI_H78A proteins (H78A in short) with the I-PpoI target site in 1p32.2 was studied using ChIP. The results (mean ± SD) represent measurements from two independent ChIP experiments. Samples were derived from three independent and nonsimultaneous transductions. No interaction with target sequence was observed in the wt IN samples studied. Results were analyzed with one-way ANOVA and Bonferroni’s multiple comparison test. ^∗∗∗𝑃<0.001; ^∗∗𝑃=0.001 to 𝑃<0.01; ^∗𝑃=0.01 to 𝑃<0.05.