Lentiviral Protein Transduction with Genome-Modifying HIV-1 Integrase-I-PpoI Fusion Proteins: Studies on Specificity and Cytotoxicity

Figure 4

A cytotoxicity study in different cell lines using two concentrations of IN-I-PpoI. Cell viabilities measured by a luminescent cell viability assay are shown as percentages of the wt IN control vector-transduced cells (mean ± SD) after a vector dose of 2 ng (a) and a vector dose of 10 ng (b) of p24. The bars represent viability after LVV mediated I-PpoI protein transduction at different time points post transduction (1, 2, 3 and 6 days, from left to right). Results were analyzed with two-way ANOVA and the Tukey’s multiple comparisons test. ^∗∗∗𝑃<0.001; ^∗∗𝑃=0.001 to 𝑃<0.01; ^∗𝑃=0.01 to 𝑃<0.05.

(a)

(b)