Evaluation of Azathioprine-Induced Cytotoxicity in an In Vitro Rat Hepatocyte System

Table 2

Effects of a ROS scavenger and various antioxidants on AZA-induced cytotoxicity in isolated rat hepatocytes.

Addition	Cytotoxicity (trypan blue uptake) (%)
Incubation time	60 min	120 min	180 min
Control hepatocytes	19 ± 1	22 ± 1	24 ± 2
+400 μM AZA	31 ± 2^a	51 ± 1^a	66 ± 2^a
+1 mM mesna	23 ± 1^b	33 ± 1^a,b	45 ± 1^a,b
+1 mM Trolox	24 ± 1^b	35 ± 2^a,b	43 ± 1^a,b
+200 μM TEMPOL	24 ± 1^b	38 ± 2^a,b	49 ± 2^a,b
+2 μM DPPD	26 ± 1^a	37 ± 1^a,b	47 ± 1^a,b

Data are presented as mean ± SEM (𝑛=3); all modulating agents were noncytotoxic compared to control hepatocytes at concentrations used. Refer to Section 2 for a description of the experiments performed and experimental conditions. Mesna, 2-mercaptoethanesulfonate; Trolox, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid; TEMPOL, 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl; DPPD, N,N′-diphenyl-p-phenylenediamine. aSignificant compared to control (only hepatocytes); bsignificant compared to 400 μM AZA.