Macrophage Capping Protein CapG Is a Putative Oncogene Involved in Migration and Invasiveness in Ovarian Carcinoma
Figure 2
(a) Relative CapG expression of ovarian cancer cell lines SK-OV-3 and Hey, prior to and after alteration of CapG expression using CapG mediated siRNA (SK-OV-3 + siCapG) and stable retroviral transfection (Hey CapG), respectively. Values were calculated performing qRT-PCR in duplicates, repeated three times. Each probe was normalized to the housekeeping gene GAPDH. (b) Effects were confirmed by Western blot analyses. Equal detection of -tubulin in samples verified equality of protein quantity. (c, d) Performing a migration assay (scratch assay), the motility in CapG siRNA treated cells was nearly halved in 24 hrs while overexpression in Hey cells led to a significantly earlier closure of the scratch after 72 hrs. (e, f) The invasiveness of siRNA treated cells was also significantly decreased in the transwell Matrigel invasion assay (by 4-fold) in SK-OV-3 cells.
