Functional Interactions between 17β-Estradiol and Progesterone Regulate Autophagy during Acini Formation by Bovine Mammary Epithelial Cells in 3D Cultures
Figure 5
Real-time PCR analysis showing the effects of (a) 17β-estradiol (1 nM), (b) progesterone (5 ng/mL), or (c) both steroid hormones administered simultaneously on the expression of chosen autophagy-related genes: ATG5, BECN1, ATG3, and LC3B in BME-UV1 cells cultured on Matrigel for 3, 6, 9, and 14 days; the levels of analyzed mRNA were normalized according to the relative GAPDH mRNA expression of each sample; the expression of each ATG under control conditions was appointed as 1 at each time point; a change in expression was considered significant () when at least 1.5-fold increase was obtained.
