Nerve Injury-Induced c-Jun Activation in Schwann Cells Is JNK Independent

Figure 3

ATF-3 is upregulated in sciatic nerve Schwann cells following axotomy. (a) ATF-3 immunopositive nuclei in the distal rat sciatic nerve segment in vivo 48 h after axotomy (to the left on the specimen). Scale bar = 500 μm. The insert shows double staining of ATF-3 (green) and S-100 (red); scale bar = 100 μm. (b) ATF-3 immunoreactivity 10 min to 48 h after axotomy in vivo. The immunopositive nuclei are expressed as a percentage of the total number of nuclei in the nerve sections. Black bars show contralateral control nerve segments, white bars show proximal nerve segments, and grey bars show distal nerve segments. Mean values ± SEM, 𝑛=5. ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001.

(a)

(b)