Research Article

ELFN1-AS1: A Novel Primate Gene with Possible MicroRNA Function Expressed Predominantly in Human Tumors

Figure 2

Identification of the primary structure of the Hs.633957-specific RNA. (a) Scheme of the transcript variants for the locus Hs.633957. Splice sites and polyadenylation sites are marked and their positions are given according to the leftmost TSS. Arrows indicate position of the primers which were used for identification of the major splice form. BX119057-like variant is filled in dark grey. (b) Results of cDNA PCR amplification with primers bordering the 39 to 3642 gene region. (c) Results of cDNA PCR amplification with primers bordering the 39–3289 gene region. cDNA samples: 1: gallbladder, adenocarcinoma; 2: rectum, well differentiated adenocarcinoma; 3: ureter, papillary transitional cell carcinoma; 4: T-cell Hodgkin’s lymphoma; 5: kidney; 6: liver; NTC: no template control.
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