RhoA Controls Wnt Upregulation on Microstructured Titanium Surfaces

Figure 4

Effects of 0.2 mM Cytochalasin treatment on (a) total RhoA activation levels by G-LISA assay (^∗𝑃<0.01 versus vehicle-treated polished; ^#𝑃<0.05 versus vehicle-treated Polished) in MC3T3 cells and (b) canonical WNT activity by Luciferase reporter assay in C2C12 cells growing on Polished or SLA titanium surfaces. ^∗𝑃<0.01 versus vehicle-treated Polished. (c, d) Real-time PCR analysis of Alkaline Phosphatase (ALP, ^∗,#𝑃<0.01 versus vehicle-treated Polished) and Osteocalcin (OCN, ^∗𝑃<0.01 versus vehicle-treated SLA) expression in murine osteoblastic MC3T3 cells on Polished or SLA surfaces after Cytochalasin addition.

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