Effect of Exercise Intensity on Isoform-Specific Expressions of NT-PGC-1α mRNA in Mouse Skeletal Muscle
Figure 5
Ectopic expression of NT-PGC-1α-a protein in C2C12 myotubes. (a) No expression of endogenous NT-PGC-1α protein in differentiated C2C12 myotube cells induced by 10−5 M AICAR for 3 h and 10−5 M clenbuterol for 1 h. Control cells were treated with vehicle. (b) Expression of exogenous NT-PGC-1α-a protein in C2C12 myotube cells by transfection with plasmid NT-PGC-1α-a/pcDNA3.1. Control cells were transfected with pcDNA3.1 empty vector. Western blot analysis of NT-PGC-1α-a using an antibody raised against the N-terminal region of PGC-1α (aa 1–300). β-Tubulin was blotted as a loading control.
