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BioMed Research International
Volume 2014 (2014), Article ID 403639, 4 pages
http://dx.doi.org/10.1155/2014/403639
Research Article

Tissue Factor and Tissue Factor Pathway Inhibitor in Chronically Inflamed Gallbladder Mucosa

1Department of Histology and Embryology, Medical University of Silesia in Katowice, Jordana Street 19, 41-808 Zabrze, Poland
2Municipal Hospital, Chełmońskiego Street 28, 43-600 Jaworzno, Poland
3Department of Pathomorphology, Medical University of Silesia in Katowice, 3-go Maja Street 13-15, 41-800 Zabrze, Poland
4Department of Biochemistry, Medical University of Silesia in Katowice, Jedności Street 8, 41-200 Sosnowiec, Poland
5Department of Descriptive and Topographic Anatomy, Medical University of Silesia in Katowice, Jordana Street 19, 41-808 Zabrze, Poland

Received 1 December 2013; Revised 19 January 2014; Accepted 21 January 2014; Published 27 February 2014

Academic Editor: Saulius Butenas

Copyright © 2014 Jacek Liczko et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We characterised a tissue factor (TF) and tissue factor pathway inhibitor (TFPI) expression in relation to severity of inflammatory infiltration of the gallbladder mucosa in a chronic cholecystitis. We prospectively studied the gallbladder specimens obtained from 54 patients who had undergone cholecystectomy due to chronic calculous cholecystitis and 16 calculosis-free gallbladder specimens obtained from patients who underwent cholecystectomy due to the polyp/polyps as well as in cases of gallbladder injury. To assess TF and TFPI immunoreactivity by immunohistochemistry, the monoclonal anti-human TF and TFPI antibodies were used. The inflammatory infiltration of the gallbladder mucosa was reflected by the number of CD3 and CD68 positive cells. The expression of TF and TFPI differed significantly between the cholecystitis and the control group. Most capillary endothelial cells of the cholecystitis group presented weak expression for TFPI. The mean number of CD3 positive lymphocytes in the cholecystitis group was 18.6 ± 12.2, but the mean number of CD68 positive cells was 29.7 ± 13.9. In the control sections, it was 3.1 ± 1.9 and 8.8 ± 3.9, respectively ( ). The results of the current study suggest that the tissue procoagulant state found may be engaged in the etiopathogenesis of the cholecystitis.