Supplementation with Sodium Selenite and Selenium-Enriched Microalgae Biomass Show Varying Effects on Blood Enzymes Activities, Antioxidant Response, and Accumulation in Common Barbel (Barbus barbus)

Table 1

Formulation, mean approximate composition, and gross energy of experimental diets.

Ingredients (g kg^−1)
Casein	320.0
Gelatine	80.0
Dextrine	280.0
Cellulose	190.0
Rapeseed oil	60.0
Carboxymethyl cellulose	20.0
Vitamin and mineral premix^a	41.61
Microalgae biomass^b	8.39
Proximate composition (g kg^−1 dw)
Dry matter	888.1
Crude protein	336.4
NFE^c	474.6
Crude lipid	21.4
Crude fibre	139.6
Ash	28.0
Gross energy (MJ kg^−1)^d	16.9

aComposition of the vitamin and mineral premix (Roboran H) provided by the manufacturer: vitamin A, 500 000 IU; vitamin D3, 100 000 IU; vitamin K, 20 mg kg⁻¹; vitamin E, 750 mg kg⁻¹; vitamin B1, 25 mg kg⁻¹; vitamin B2, 40 mg kg⁻¹; vitamin B6, 20 mg kg⁻¹; vitamin B12, 0.5 mg kg⁻¹; niacin, 200 mg kg⁻¹; calcium pantothenate, 200 mg kg⁻¹; choline chloride, 20 000 mg kg⁻¹; biotin, 10 mg kg⁻¹; cobalt as CoSO₄·7H₂O 6 mg kg⁻¹; copper as CuO 150 mg kg⁻¹; iodine as KI 20 mg kg⁻¹; iron as FeSO₄·H₂O 380 mg kg⁻¹; manganese as MnO 110 mg kg⁻¹; zinc as ZnO 140 mg kg⁻¹.
^b Composition of Se-free and Se-enriched algal biomass differed among treatments (see Table 2).
^c Nitrogen-free extract, NFE = 1000 − (protein + lipid + ash + crude fibre) (g kg⁻¹).
^d Calculated assuming conversion factors of 23.6, 39.5, and 17.2 MJ kg⁻¹ for protein, lipid, and NFE, respectively [68].