Periodontal Ligament Mesenchymal Stromal Cells Increase Proliferation and Glycosaminoglycans Formation of Temporomandibular Joint Derived Fibrochondrocytes

Figure 1

PD-MSCs increase proliferation and GAG formation of TMJ-chondrocytes. (a) EdU staining and Alcian Blue staining of monoculture and coculture pellets. At day 3 after aggregation, EdU staining was performed to detect proliferating chondrocytes. EdU positive cells are visualized by Alexa 594 (red), as indicated by white arrowhead. PD-MSCs are marked by GFP (green), and nuclei were counterstained with Hoechst 33342 (blue). At day 21 after aggregation, GAGs were stained by Alcian Blue. Fibrotic tissue was indicated by asterisk. Scale bar = 100 μm. (b) Quantification of EdU positive chondrocytes. Quantification is based on the assumption that all non-green cells are TMJ chondrocytes. Data from 3 donor pairs were calculated to show deviation. Statistical significance was analyzed by Student’s t-test. (c) GAG quantification. Quantitative GAG assay shows more GAGs in mixture group than in the other two groups at week 3 after aggregation. Error bar reflects standard deviation. values were calculated with one-way ANOVA followed by Dunnett’s test.

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