Periodontal Ligament Mesenchymal Stromal Cells Increase Proliferation and Glycosaminoglycans Formation of Temporomandibular Joint Derived Fibrochondrocytes

Figure 2

PD-MSCs disappear after coculture. (a)  Fluorescent images of pellets at 21 days after aggregation. Cryosections were made without fixation. Scale bar = 100 μm. Arrowhead indicates remaining green cells in coculture pellets. (b) Fluorescent images were analyzed to quantify GFP positive cells. Data from 3 donor pairs were calculated to show deviation. (c) Quantitative PCR of GFP. GAPDH was amplified on genomic DNA to stand for cell numbers. GFP was amplified and normalized to GAPDH. PD-MSC was chosen as reference. Number in mixture group represents the relative amount of GFP compared to PD-MSC. Three donor pairs were analyzed.

(a)

(b)

(c)