Periodontal Ligament Mesenchymal Stromal Cells Increase Proliferation and Glycosaminoglycans Formation of Temporomandibular Joint Derived Fibrochondrocytes

Figure 3

Conditioned medium increases proliferation and GAG deposition. (a)  EdU staining was performed at day 3 after aggregation. Positively stained cells are visualized by Alexa 594 (red), as indicated by white arrowhead. Nuclei were counterstained with Hoechst 33342 (blue). Scale bar = 100 μm. (b)  EdU positive cells were quantified based on fluorescent images . Data is shown as deviation. Statistical significance was analyzed by Student’s t-test. (c)  Alcian Blue staining was carried out at day 21 after aggregation for GAGs. Scale bar = 100 μm. (d)  GAG contents in pellets were assayed at day 21 after aggregation . Error bar reflects standard deviation. values were calculated with Student’s t-test.